Aloha Friday gang! Seems like these months since I started this blog are just flying by at light speed. I personally have discovered and learned an enormous amount of incredible information on plants and horticulture, and I’m still excited to learn more…..With all that being said, I have been driven by an insatiable passion to learn some advanced science in horticulture. Here is a great video from Virginia Tech on molecular plant sciences. This is exactly the field I have been going towards. Check it out.

Happy Aloha friday gang! Lets get into some hardcore science today. Today’s topic is Micro-Propagation / Plant Tissue Culture.

If you have ever wondered what kind of plant science you would be doing if you were in a university program for Horticulture, BAM, this is it. I recently discovered this technique because we had a plant tissue kit that was begging for someone to use it. My curiosity got the best of me and later that night I found myself deep in the throws of a full on academic endeavor. All I can say is, after 20 years of taking cuttings, our community now needs this level of precision, technology, and science. I am so excited about this knowledge and I look forward to sharing it with you guys now.

What is Micropropagation?
Micropropagation is a way to clone plants axenically (sterile). Terminal buds collected from branches of mature plants are surface sterilized and placed in test tubes containing a specific nutrient medium. The different media we are experimenting with usually contain a carbon source, vitamins, plant hormones and antibiotics. Plantlets grown from buds of the same plant, barring mutation, will be clones (i.e., genetic replicates) of the plant from which they were cut. Once we are able to maintain rapidly multiplying plant stocks in the lab, we can use them as a source for additional micropropagation or subculturing. Subculturing involves dividing sterile plantlets into smaller segments and growing plants from these pieces. This is how we grow Widgeon-grass (Ruppia maritima) in the lab, and we are in the process of developing this technique for Shoal-grass (Halodule wrightii). Widgeon-grass is the easiest seagrass to micropropagate; other species, such as Shoal-grass (Halodule), Manatee-grass (Syringodium filiforme) and Turtle-grass (Thalassia testudinum), are increasingly difficult. We are focusing on one species at a time.

Why Use Micropropagation?
Compared to standard nursery techniques, micropropagation has the potential to produce more plants in less time. Fewer stock plants are needed because an explant can supply a great amount of material from which clonal material can be subcultured. Also, not having to rely on seeds and other propagation methods ensures greater uniformity in plants produced. Clones can be screened for different attributes and used for specific applications, or genetic variability can be assured by mixing different clonal strains in restoration projects. Another advantage to micropropagation is the contamination-free condition of the plants produced and the ability to produce microbially fit stocks that resist pathogens.

We have plant tissue kits available, give us a call 1-866-PGS-GROW